Composite

Part:BBa_K3482014

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)
Revision as of 16:51, 26 October 2020 by Santosmoreno j (Talk | contribs) (→‎Biology and Usage)


aTc-inducible IM2 antitoxin

IM2 antitoxin under a pTet promoter

Biology and Usage

The IM2 molecule is the immunity protein of the Colicin E2 endonuclease. The IM2 protein is here under a pTet promoter and can therefore be activated by aTc.

This part is used to create a kill-switch in combination with part BBa_K3482004 .

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482004 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 242
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None