Composite
Part:BBa_K3407019:Design
Designed by: Javier Navarro Delgado Group: iGEM20_TUDelft (2020-10-23)
YmdB regulator of RNAseIII in E. coli with tetA/tetR promoters - RBD - T1 terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BglII site found at 68
Illegal BamHI site found at 673
Illegal XhoI site found at 682 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
tetA/tetR - RBD - YmdB - T7t
Source
tetA/tetR - RBD - YmdB - T7t