Composite

Part:BBa_K3482042

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)
Revision as of 21:42, 25 October 2020 by Jeremy95 (Talk | contribs)


azurin with NSP4 secretion and 8His tag

Azurin protein fused with NSP4 secretion signial peptide and a 8His secretion tag.

Usage and Biology

Azurin is a blue copper-binding redox protein originally found in the periplasmic membrane of Pseudomonas aeruginosa. It has recently been discovered to have an l anticancer effect by targeting the well-known p53 apoptosis pathway and by preferentially entering cancerous cells.

We used this part with a tag for protein expression and purification


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None