Composite

Part:BBa_K3523008

Designed by: Kong Yangyang   Group: iGEM20_Xiamen_city   (2020-10-18)
Revision as of 11:45, 18 October 2020 by Registry (Talk | contribs)

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J23116-rbs-Leader sequence-SOD-rbs-katA-Membrane Protein Ag43_700

BBa_K3523008 is used to express a fusion protein, helping to display it on the cell membrane surface. The fusion protein contains superoxide dismutases (SOD) and catalase,. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2−) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2166
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2731
    Illegal AgeI site found at 2470
    Illegal AgeI site found at 2884
    Illegal AgeI site found at 3126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None