Part:BBa_K3440010

luxI-Myc under bphR1 promoter

Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)-Myc(BBa_K823036)

Usage and Biology

Expresses LuxI under bphR1 promoter. bphR1 promoter can be activated by bphR2 or the complex bphR2:PCB. LuxI produces 3OC6-HSL, a quorum sensing molecule.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.

Colony PCR gel for BBa_K3440010(M) and BBa_K3440009(L)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 963
  Illegal BglII site found at 1001
  Illegal BamHI site found at 156
  Illegal BamHI site found at 239
  Illegal XhoI site found at 46
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 139