Composite

Part:BBa_K3396009

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)
Revision as of 12:10, 25 October 2020 by FloraLiu (Talk | contribs)


GFPnano-DocS-P2A-Trim21-Coh2

CMV->Kozak->Truncated TRIM21->DocS->P2A->Coh2->GFPnano->BGH-pA

This part is designed to prove that our Trim21 truncation strategy works as we expected. Since the original Trim-away system utilizes the Trim21 PRYSPRY domain to interact with the antibody IgG, we herein changed such interaction pair into other constitutively dimerization pairs to validate our design.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1790
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


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Categories
Parameters
None