Composite

Part:BBa_K3396009

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)
Revision as of 08:28, 25 October 2020 by Registry (Talk | contribs)

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GFPnano-DocS-P2A-Trim21-Coh2

Usage and Biology The PRYSPRY-lgG Fc interaction of natural Trim21-antibody complex is replaced with DocS-Coh2 to demonstrate that the Trim-Away alike method could still work. DocS and Coh2 are a pair of proteins that has been widely used in synthetic biology to test split protein approach due to their high constitutively dimerization affinity. Theoretically, the dimerization of DocS and Coh2 will bring the truncated Trim21 protein and the target protein EGFP into proximity and form a s stable trimer. Truncated Trim21 would then function as the E3 ligase to ubiquitylate EGFP and mediate its degradation. We expect such system to work in a similar manner as the previous GFP predator as we reported in iGEM 2018. T--NUDT_CHINA--GFPnano-DocS-P2A-Coh2-Trim21_Figure1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1790
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1747
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


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