Regulatory
pTrc-2d

Part:BBa_K3332040

Designed by: Jinzhu Mao   Group: iGEM20_XMU-China   (2020-10-16)
Revision as of 07:06, 16 October 2020 by Registry (Talk | contribs)

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pTrc-2 derivative

A promoter derived from Ptrc-2 promoter can be strongly repressed by LacI protein.We use it to express mf-lon and MazF in the absence of aTc so as to inhibit the growth of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None