Intermediate

Part:BBa_K3440019

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 14:45, 24 October 2020 by JuCo (Talk | contribs)


MtrB under Lux promoter

Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-DT(BBa_B0015)-Plux(BBa_R0062)-RBS(BBa_B0034)-mtrb(BBa_K3146001)-DT(BBa_B0015)

Usage and Biology

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


[edit]
Categories
//classic/signalling/receiver
Parameters
proteinsmtrb, LuxR