Composite

Part:BBa_K3328014

Designed by: Chaoxin Chen   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 16:42, 20 October 2020 by Hester (Talk | contribs)


triggers of XOR gate (XOR1)

two input trigger of XOR1 logic gate to perform the truth table

Design

The XOR is inspired by the NIMPLY gate, it is consisted of a toehold switch and two triggers. The trigger’s core sequence is same and at the triggers’ both ends there are the nucleotide-binding domains. When input one of these triggers, the switch can turn on. And when input these two triggers at the same time, they can pair together and form a ring in the middle, as the result, the switch will still be in OFF state.

Result

800px-T--OUC-China--part_XOR1.jpg

The left figure shows that when INPUT A=1, INPUT B=0, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This didn't correspond to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None