Device

Part:BBa_K137040

Designed by: Allen Lin   Group: iGEM08_Caltech   (2008-07-21)
Revision as of 01:39, 24 October 2008 by Jkm (Talk | contribs)

Coding SSM device: GFP with (AGTC)10 after start codon

Strong promoter + strong RBS + GFP with (AGTC)10 repeat after start codon + double terminator

Usage and Biology

As part of the Caltech 2008 iGEM [http://2008.igem.org/Team:Caltech/Project/Population_Variation population variation project], Parts K137040 to K137043 were placed on pSB1A2 and transformed into DH10B. When measured on a plate reader (Tecan Sapphire), they gave the following fluorescence values:

SSM.gif

When measured on a flow cytometer using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], they produced the following distributions:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 745


[edit]
Categories
Parameters
None