Composite

Part:BBa_K3009039

Designed by: Alisa Zinina   Group: iGEM19_Freiburg   (2019-10-21)
Revision as of 03:44, 22 October 2019 by Registry (Talk | contribs)

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This is a trimolecular fusion of tRNA Synthetase and SDP5, MCP

The tyrosyl-tRNA-synthetase from M.jannashii and cognate tRNA pair are acting orthogonally to the endogenous translational machinery of E.coli. By binding and loading non-canonical amino acids onto the tRNA, the tyrosyl-tRNA-synthetase enables their incorporation into proteins. The tRNA is cognate towards the amber stop codon, which is suppressed by the non-canonical amino acid. [1] MS2 Capsid Protein(MCP), binds strongly to specific stem-loop structures derived from the MS2 phage genome. Proteins fused to MCP can be brought in close proximity to mRNA tagged with stem-loops.pindle-deficient protein 5 derived from C.elegans is able to assemble into condensates that built a separate phase from the surronding cytosol, therefore emulating a reaction compartment. Fusion proteins will also be confined to this organelle.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
    Illegal AgeI site found at 1507
    Illegal AgeI site found at 1606
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 304


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