Coding

Part:BBa_K2972005:Design

Designed by: Huanzhou Yang   Group: iGEM19_BIT-China   (2019-10-16)
Revision as of 16:04, 21 October 2019 by UOH4 (Talk | contribs) (→‎Design Notes)


GGPP synthase (crtE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 223
    Illegal AgeI site found at 96
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The crtE protein is mainly composed of a helix and an irregular coil. Its hydrophobic cavity is a key site for binding and catalysis to the substrate, and mutation of its key site is expected to increase the activity of the enzyme.

Source

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References