Device

Part:BBa_K3165037

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)
Revision as of 01:37, 21 October 2019 by Bvivan (Talk | contribs)


mini-CcaS GeneratorK This part is used as a generator for mini-CcaS (BBa_K3165000) under a constitutive promoter (BBa_J23106) and a strong ribosome site (BBa_K3165005) with a double terminator (BBa_B0015) .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain.
It is the light-sensitive part of the photo-sensitive CcaS-CcaR system. This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.


[edit]
Categories
Parameters
None