Reporter

Part:BBa_K3165006:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-18)
Revision as of 22:30, 20 October 2019 by Bvivan (Talk | contribs) (→‎Source)


ECFP (Optimized for Escherichia coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ECFP belongs to the family of Cyan Fluorescent proteins, where the Tyr-66 is replaced with a Trp. The ECFP coding sequence was developed by the mutations F64L, S65T, Y66W, N146I, M153T and V163A in the GFP sequence. The part was codon optimised for Escherichia coli.


Source

This sequence is derived from the existing part (BBa_E0020) in the iGEM registry.

References