Chlamydomonas reinhardtii chassis

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Chlamydomonas reinhardtii

Chlamydomonas reinhardtii is a green microalga. The unicellular and genetically modifiable organism posses several characteristics making it an interesting model organism for synthetic biology.

  • Inexpensive & easy cultivation
  • Phototrophic
  • Enviromental-safe
  • Two expression compartments (nucleus & chloroplast)
  • Post-translational modification
  • one organism = single cell
Fig. 1. Raster electron microscope image of C. reinhardtii
Fig. 2. Schematic overview of C. reinhardtii

References

[1] Harris, E. H. (2001). "CHLAMYDOMONAS AS A MODEL ORGANISM." Annu Rev Plant Physiol Plant Mol Biol 52: 363-406.

[2] Sizova, I., Fuhrmann, M., & Hegemann, P. (2001). A Streptomyces rimosusaphVIII gene coding for a new type phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene, 277(1-2), 221-229.