Reporter

Part:BBa_K1696005

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-11)
Revision as of 14:07, 17 September 2015 by Ggjyliuyue (Talk | contribs) (Reference)

P170 Promoter

P170 is upregulated at low pH (6.0~6.5) during the transition to stationary phase. The minimal DNA region required for both promoter activity and pH regulation was mapped to a 51 bp fragment located 7 bp upstream of the transcriptional start site. This fragment lacked the consensus -35 promoter region, but it contained an ‘extended’ -10 promoter region[1]. Besides, a novel 14 bp regulatory DNA region centered at around -41.5 and composed of three tetra nucleotide sequences, boxes A, C and D. Boxes A and C contribute to P170 activity, whereas box D and the position of boxes ACD (renamed ACiD-box) are essential to P170 activity and acid response[2].


Reference

[1] Madsen S M, Arnau J, Vrang A, et al. Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis.[J]. Molecular Microbiology, 1999, 32(1):75-87.

[2] Søren M. Madsen, Thomas Hindré, Jean-Paul Le Pennec, et al. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD-box and the transcription regulator RcfB[J]. Molecular Microbiology, 2005, 56(3):735-746.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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