Composite

Part:BBa_K2969050

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)
Revision as of 10:41, 16 October 2019 by Registry (Talk | contribs)

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J23119-CI434ts-TEVsite-sfGFP

This part contains the coding gene of the thermo-sensitive transcription factor CI434ts-TEVsite under the constitutive promoter J23119 and a reporter gene sfGFP under the promoter of CI434ts. When the CI434ts is inactivated as the temperature changes, the inhibition of sfGFP expressing will be erased.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 754


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Categories
Parameters
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