Device
Dual inver

Part:BBa_K3093100:Design

Designed by: Miao Zongjie; Jin Qianwei   Group: iGEM19_ECUST_China   (2019-10-16)
Revision as of 08:09, 17 October 2019 by Still Water (Talk | contribs) (→‎References)


PlacIq-LacI-Plac-cI-λPR dual inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3156
    Illegal AgeI site found at 3268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The inverter system consists of lactose operon, CⅠprotein and PλR012. The LacI repressor is constitutively expressed which inhibites transcription from the pLac promoter in the absence of IPTG, thus PλR012 could be active. While the expression of the CI repressor can be induced by externally added IPTG and inhibited the transcription of PλR012, hence realizing the reversal of the function.

Source

The source of dual inverter was almost from iGEM part.

References

[1]Yokobayashi Y, Weiss R, Arnold FH. Directed evolution of a genetic circuit. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16587-91. Epub 2002 Nov 25. PubMed PMID: 12451174; PubMed Central PMCID: PMC139187.