Coding
Part:BBa_K3013003
Designed by: Gereon Fischer Group: iGEM19_Aachen (2019-10-13)
mamC-FbFP BS2 fusion
This fusion protein consists of mamC a glycine linker and a fluorescent protein. MamC is suggested to be located in the magnetosome membrane, wich are produced by magnetotactic bacteria. To report expression of mamC FbFP BS2 is used. This part is an improvement of part BBa_K1094401, because magnetotacic bacteria will produce magnetozomes under anaerobic conditions. In this case there is the need of an anaerobic reporter, as eGFP is dependent to oxygen to develope fluorescence. FbFP BS2 (BBa_K3013002) is flavin mononucleotide-based an independent to oxygen. The glycine linker will help the correct folding of mamC and FbFP BS2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 498
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 169
Illegal NgoMIV site found at 253
Illegal AgeI site found at 666 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//cds/membrane
//cds/reporter
//function/reporter/fluorescence
//cds/membrane
//cds/reporter
//function/reporter/fluorescence
biology | |
emission | |
excitation | |
protein |