DNA
Part:BBa_K2912013
Designed by: Lingling Liao Group: iGEM19_SZU-China (2019-10-13)
M. micrantha_leaves_ Unigene0033458
This is a single-stranded DNA that can be cyclized by T7 promoter, since it has two sites complementarty to T7 promoter. It can transcribe RNA interference (RNAi) molecule by rolling circle transcription, which can silence the gene encoding chlorophyll A-B binding family protein LHCA5 of M. micrantha, through which we can block this essential matabolic gene expression to kill such invasive weed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |