Part:BBa_K3187022

Superfolder Green Fluorescence Protein

Profile

Name	sfGFP (superfolder green fluorescent protein)
Base pairs	714
Molecular weight	26.8 kDa
Origin	Aequorea victoria
Properties	very fast maturing variant of GFP

Usage and Biology

GFP is a fluorescent protein originally isolated from the jellyfish Aequorea victoria. sfGFP is a variant of this protein. It has a short maturing time of 13.6 min instead of 36.1 min of the original protein variant, an extinction maximum at 485 nm and an emission maximum at 510 nm ^[1] ^[2] ^[3].

References

↑ Jean-Denis Pédelacq, Stéphanie Cabantous, Timothy Tran, Thomas C Terwilliger, Geoffrey S Waldo, Engineering and characterization of a superfolder green fluorescent protein, Nature Biotechnology volume24,pages79–88 (2006) [1]
↑ FPbase: Superfolder GFP, last visited: 6.10.2019 [2]
↑ Balleza, E., Kim, J. M., & Cluzel, P. (2018). Systematic characterization of maturation time of fluorescent proteins in living cells. Nature methods, 15(1), 47–51. [3]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 10

[1] Jean-Denis Pédelacq, Stéphanie Cabantous, Timothy Tran, Thomas C Terwilliger, Geoffrey S Waldo, Engineering and characterization of a superfolder green fluorescent protein, Nature Biotechnology volume24,pages79–88 (2006) [1]

[2] FPbase: Superfolder GFP, last visited: 6.10.2019 [2]

[3] Balleza, E., Kim, J. M., & Cluzel, P. (2018). Systematic characterization of maturation time of fluorescent proteins in living cells. Nature methods, 15(1), 47–51. [3]

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