Part:BBa_K2936011
light-dependent controlled lysis system
In order to be biologically safe, bacterial lysis is essential. This part can be easily used to achieve the lysis of bacteria under light conditions. In this system, under dark conditions, phosphorylated FixJ protein was transferred from YF1 protein to FixJ protein, and phosphorylated FixJ protein activated pFIXK2 promoter, then activated downstream gene expression.When the LacI protein binds to LacO, it prevents the downward expression of Lac promoter, the lysis gene is not activated and the cells grow normally. When cultured under light conditions,phosphorylation of FixJ protein was blocked and gene expression regulated by pfixk2 promoter was inhibited. Lac promoter expression was not inhibited, lysis gene expression was normal,then we achieve the lysis of bacteria. When using this system,you just need introduce the plasmid to the host cell.Then you could achieve the lysis of bacteria after you turn on the light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
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