Plasmid

Part:BBa_K2908681:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-17)
Revision as of 18:41, 17 October 2019 by Registry (Talk | contribs)

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s(ESR1)p-miR101spe-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 155
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initiation codon ATG can only appear between miRNA sponse and EGFP.


Source

We designed it by ourselves and constructed this part with the help of Golden gate.

References