Coding

Part:BBa_K3279008:Design

Designed by: Liang Siwen   Group: iGEM19_CAU_China   (2019-10-15)
Revision as of 15:27, 15 October 2019 by SvenL (Talk | contribs)


cenA gene fused with INP-N sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 408
    Illegal AgeI site found at 1051
    Illegal AgeI site found at 1414
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primary concern was BioBrick compatibility so that illegal sites in RFC[10] did not appear in the sequence. And the sequeces was finally designed to link with pET30a(+) , so we added restriction enzyme cutting site NdeI at the 5' and SalI at the 3'.


Source

cenA gene (BBa_K118023) is from Cellulomonas fimi genomic DNA. INP-N sequence (BBa_K3279006) is originally from Pseudomonas syringae genomic DNA and we (CAU_China 2019) did a codon optimization.

References

[1]Gu, M. Z., Wang, J. C., Liu, W. B., Zhou, Y. & Ye, B. C. Expression and displaying of beta-glucosidase from Streptomyces coelicolor A3 in Escherichia coli. Applied biochemistry and biotechnology 170, 1713-1723, doi:10.1007/s12010-013-0301-4 (2013).

[2] Li Mingya, Lin Chenshui, Li Mingya, et al. Ice-nucleation Protein and Its Application in Bacterial surface Display Technology [J]. Amino Acids and Biological Resources, 2016, 38 (2): 7-11.