Part:BBa_K3102029:Design
T7-RBS-Riboflavin-Ter
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1013
Illegal BglII site found at 2110 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 354
Illegal PstI site found at 3318
Illegal AgeI site found at 201
Illegal AgeI site found at 903
Illegal AgeI site found at 945
Illegal AgeI site found at 1488
Illegal AgeI site found at 1863 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331
RibB : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465
RibD : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321
RibE : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322
RibC : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
"Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli." Foor F., Brown G.M. J. Biol. Chem. 250:3545-3551(1975)
"Riboflavin synthases of Bacillus subtilis. Purification and properties." Bacher A., Baur R., Eggers U., Harders H.D., Otto M.K., Schnepple H. J. Biol. Chem. 255:632-637(1980)
"Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli." Eberhardt S.M.R., Richter G., Gimbel W., Werner T., Bacher A. Eur. J. Biochem. 242:712-719(1996)