Coding
Part:BBa_K3102000:Design
Designed by: Chloe DOIZELET Group: iGEM19_Ionis_Paris (2019-10-02)
RibA (coding for GTP cyclohydrolase-2)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 319
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 319
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 319
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 319
Illegal AgeI site found at 166 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331
References
"Purification and properties of guanosine triphosphate cyclohydrolase II from Escherichia coli." Foor F., Brown G.M. J. Biol. Chem. 250:3545-3551(1975)