Composite

Part:BBa_K3102025:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 09:47, 12 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎Design Notes)


T7-RBS-TEVp-ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 847
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TEvp:Sequence synthesized and optimized for E.coli on Geneius

Source

None

References

Phan, J., Zdanov, A., Evdokimov, A. G., Tropea, J. E., Peters, H. P. K., Kapust, R. B., Li, M., Wlodawer, A., and Waugh, D. S. (2002). Structural basis for the substrate specificity of tobacco etch virus protease. J. Biol. Chem. 277: 50564-50572.