Coding

Part:BBa_K3102021:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 18:02, 7 October 2019 by SDUQUESNE (Talk | contribs) (→‎References)


ACS (Acetyl-coenzyme A synthetase) Escherichia.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 802
  • 1000
    COMPATIBLE WITH RFC[1000]


=Design Notes

C1563T because Esp3I GGA

Source

This sequence comes from E.coli and was found through Biocyc:

https://biocyc.org/gene?orgid=ECOLI&id=EG11448

References

Brown TD. et al., The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli, J Gen Microbiol. 1977 Oct;102(2):327-36.

Kumari S. et al., Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli, J Bacteriol. 1995 May;177(10):2878-86.