Coding

Part:BBa_K3102014:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 14:43, 7 October 2019 by SDUQUESNE (Talk | contribs) (→‎References)


LldP (L-lactate permease)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

This sequence comes from E.coli and was found through Trypanocyc: http://vm-trypanocyc.toulouse.inra.fr/ECOLI/sequence?type=GENE&object=EG11961

References

Dong JM et al., Three overlapping lct genes involved in L-lactate utilization by Escherichia coli., Journal of Bacteriology, 1993 Oct;175(20):6671-8. Nunez MF et al., Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli, Biochem Biophys Res Commun. 2002 Jan 18;290(2):824-9.