Protein_Domain

Part:BBa_K112201:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)
Revision as of 23:30, 3 September 2008 by Sixpi (Talk | contribs) (→‎Design Notes)

< T4 holin! in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112001

bx3 Forward biobricking of {<T4 Holin!} ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc bx2 Reverse biobricking of {<T4 Holin!} cgttaGGATCCttatttagcccttcctaatattctggccgcc

Source

References