Composite

Part:BBa_K3142002

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-09-28)
Revision as of 02:24, 29 September 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Acid-induced promoter and repressor lacR of lac operon

Effectively secretes proteins from lactic acid bacteria to the outside of the cell.The secretory signal peptide of the secreted protein usp45 gene of Lactococcus lactis MG1363 and the coding sequence of the first 11 amino acids of the mature peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989
    Illegal SapI.rc site found at 718


[edit]
Categories
Parameters
None