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Part:BBa_K2973015:Design

Designed by: Aphrodite Katsaouni, Nick Delkis   Group: iGEM19_Thessaly   (2019-09-28)
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Hepatitis B Virus fragment (281-560bp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose this specific fragment from the HBV genome, due to the availability of specific primer sets in the published literature.


Source

NCBI Hepatitis B Virus Genome, Strain ayw, Accession Number: NC_003977.2

References