Reporter

Part:BBa_I13522

Designed by: jkm   Group: iGEM04_MIT   (2005-07-01)
Revision as of 00:10, 27 October 2007 by Ben Yi (Talk | contribs)

pTet GFP

Untagged GFP behind a constitutive promoter.

Usage and Biology

GFP only control

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Pictures

  • BBa_I13522 visualized under non-UV lightbox

  • BBa_I13522 visualized under 254nm wavelength UV lightbox

Characterization of Cell-Free Chassis

This part was used to characterize the cell-free chassis. Details of characterization can be found on the cell-free characterization page. Other interesting isothermal properties of this construct are available at the [http://parts.mit.edu/igem07/index.php/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.

Parameter Value and Description
Optimal Temperature Expression increases with temperature from 4°C to 30°C
Optimal DNA Concentration Works optimally with 2µg of DNA in 60 µl of cell-extract
Peak Expression Time Peak rate of output occurs after approximately 85 minutes
Expression Lifespan Expression levels off after 2 hours
Expression Capacity Can synthesize over 200pmols of GFPmut3b in 6 hours
[edit]
Categories
//classic/reporter/pret
Parameters
emissionGFP
excitation
tagNone