Part:BBa_K3168006
Cysteine-free-NanoLuc
NanoLuc is a deep-sea shrimp derived luciferase, which is smaller compared to the Firefly and Renilla luciferases and therefore offers certain advantages over the traditional methods. NanoLuc has an increased stability, a smaller size and a >150-fold increase in luminescence (England, 2016). Furthermore, NanoLuc displays high physical stability, maintains its activity during incubation up to 55 oC or in culture medium for >15 h at 37 oC and shows in mammalian cells no evidence of posttranslational modifications or subcellular partitioning (Hall, 2012). The substrate for NanoLuc, called Furimazine shows increased stability and lower background activity, this enhances the possibilities for bioluminescence imaging (England, 2016). Furimazine reacts with NanoLuc in the presence of oxygen. Furimazine is converted to Furimamide and a blue luminescence output occurs. Furthermore, a flexible (SGG)2 linker is located in front of NanoLuc to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |