Reporter

Part:BBa_K3128004:Design

Designed by: Lucas PINERO   Group: iGEM19_Grenoble-Alpes   (2019-07-08)
Revision as of 08:25, 6 August 2019 by Pinerol (Talk | contribs) (→‎Design Notes)


NanoLuciferase reporter for BACTH assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the BBA J04450 and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.

Source

PLac-RBS and the termintors are from iGEM plates from Bba_J04450 The NanoLuc gene is from Promega vector's

References