Composite

Part:BBa_K2624005:Experience

Designed by: Shuyao Zhou, Huandi Xu, Junqing Zhang, Yali Wu   Group: iGEM18_CPU_CHINA   (2018-08-17)
Revision as of 03:46, 8 December 2018 by KKehuandiCPU (Talk | contribs) (→‎Applications of BBa_K2624005)


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Applications of BBa_K2624005

We used this part to construct the "p-H" Plasmid and "Plasmid2", in order to verify the role of RNA interference caused by pri-miRNA. According to the results of qPCR, the decreased MAP4K4 mRNA expression was observed. What's more, the results of MTT shows that the regulatory system we built can work.

20181017211354%21T--CPU_CHINA--hp-qidongzi.png

Figure 1. The expression of MAP4K4 mRNA

"p-H" is "pcDNA-HULC--primiRNA(MAP4K4)" plasmid;"Plasmid1" is"pIRES-tTA-NS5B-NLS";"Plasmid2" is"pcDNA-HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge".

20181017212106%21T--CPU_CHINA--hp-qidongzi.png

Figure 2. The MTT Assay

"Plasmid1" contains "tTA-NS5B-NLS" parts; "Plasmid2" contains "HULC--primiRNA(MAP4K4)-U6-pSil-TRE-sponge" parts; TC is the abbreviations of Tetracycline.

User Reviews

UNIQfd998fcd3f77e8a7-partinfo-00000000-QINU UNIQfd998fcd3f77e8a7-partinfo-00000001-QINU