Composite

Part:BBa_K2868024

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)
Revision as of 17:13, 9 October 2018 by Registry (Talk | contribs)

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csgA:CBD Expression system

T7 promoter with RBS, followed by a SalI cut-site, then coding region for csgA, followed by a flexible linker, followed by the NEB chitin binding domain, which is followed by the multi-tag of Flag, lumio, and 6x polyhistidine, and a stop codon, then a BglII cut-site, and finally a T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None