Composite

Part:BBa_K2868028

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)
Revision as of 00:54, 13 October 2018 by Cale (Talk | contribs)


4xCBD Fusion Protein Expression System

T7 promoter, a salI cut-site, then a start codon then a Flag, Lumio, 6x polyhisitine tag, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, then another linker, followed by Bacillus circulans chitin binding domain, flexible linker, S. griseus chitin binding domain, stop codon, a bglII cut-site, and finally t7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 809
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None