Composite

Part:BBa_K2675056

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-07)
Revision as of 23:12, 17 October 2018 by ELebrun (Talk | contribs)


sfGFP-LVAtag expression casette under the control of J23110 promoter

This part is an sfGFP-LVAtag (BBa_K2675006) expression cassette under the control of the constitutive promoter BBa_J23110.

Usage and Biology

This part is sfGFP-LVAtag (BBa_K2675006) was equipped by a custom made RBS (BBa_K2675017) and placed under the control of the constitutive promoter (BBa_J23110).

The pictures of E. coli cells harbouring this part show a very strong sfGFP-LVAtag expression by this this part BBa_K2675056 and no sfGFP-LVAtag in the absence of the promoter and the RBS (BBa_K2675081).

T--Evry Paris-Saclay--Drops-on-plate BBa K2675081.png

Figure 1. Pictures of E. coli cells harbouring an empty pSB1C3 backbone, BBa_K2675081 or this part BBa_K2675056.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 90
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None