RNA
crRNA

Part:BBa_K2627003:Design

Designed by: Zhaoqin Zhang   Group: iGEM18_SDU-CHINA   (2018-10-06)
Revision as of 08:56, 6 October 2018 by Registry (Talk | contribs)

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crRNA targeting GltA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We deleted the restriction site for EcoRI by Gibson Assembly.


Source

Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).

References