Composite
SAHS 2

Part:BBa_K2623016

Designed by: Yuanjun Shang   Group: iGEM18_XMU-China   (2018-09-28)
Revision as of 02:21, 14 October 2018 by Bb1984 (Talk | contribs) (→‎SUMMARY)


Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator)

Summary

TDP circuit is used to express exocrine protein SAHS. We added amilCP BBa_K592009 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 and an RBS BBa_B0034, and followed by a double terminator BBa_B0015.

CAHS_Fig1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
    Illegal XhoI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 558
    Illegal SapI.rc site found at 553


[edit]
Categories
Parameters
None