RNA

Part:BBa_K2611000

Designed by: Yuanhan Yang   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 05:45, 17 October 2018 by Registry (Talk | contribs)

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sgRNA (spacer J23101-GFP)

This sgRNA targets part "spacer J23101-GFP"(BBa_K2611001), and can combines with it's spacer sequence. This sgRNA here can combine with dCas9 protein to direct the complex to the upstream sequence of promoter of GFP to prevent transcription initiation. So, we suggest to use both this part and the GFP we have already modified (BBa_K261101).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None