Composite
Part:BBa_K2686005:Experience
Designed by: Thomas Jordan Group: iGEM18_EPFL (2018-10-07)
The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing. The part was then amplified out of the pET vector and inserted into the pSB1C3 plasmid backbone, and submitted. There was not enough time to perform Sanger sequencing, but colony PCR gave us the correct insert size.
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how you used this part and how it worked out.The part was designed and constructed in pET vector. The sequence was then confirmed on pET vector using sanger sequencing.
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