Composite

Part:BBa_K2686005

Designed by: Thomas Jordan   Group: iGEM18_EPFL   (2018-10-07)
Revision as of 13:16, 7 October 2018 by Registry (Talk | contribs)

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Encapsulin protein with HexaHistidine insert & sfGFP under native promoter included between BsaI cut

This Encapsulin protein has been modified as to have a cut site for BsaI at the C terminus. This allows for the rapid, scarless introduction of antigen peptides which will be expressed on the surface of the subunit. The insert in between the two BsaI cut sites consists of sfGFP with a native promoter and terminator, which allows for checking the success of the insertion (green colonies do not contain the desired peptide insert).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 426
    Illegal SapI.rc site found at 457
    Illegal SapI.rc site found at 1031


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Parameters
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