Composite
Part:BBa_K2549031
Designed by: Rongrong Du Group: iGEM18_Fudan (2018-10-09)
8*ZF43.8-CMV
This part is one of the response elements of our amplifier, also executing the combiner function. 8*ZF43.8 binding sites is assembled using two 4*ZF43.8 binding sites (Part:BBa_K2446006) with a biobrick scar between them. CMV (Part:BBa_K2549050) is a promotor which has a high-level constitutive expression. This part can switch off the expression of gene downstream after induced by our zinc finger-based transcription repressor.
Biology
synthetic promotor operators regulated by artificial zinc finger-based transcription factors
JJ Collins et al have reported several synthetic promotor operators which can interact with artificial zinc finger-based transcription factors with high specificity and high orthogonality[1].
JJ Collins et al stated:sTFs constructed from OPEN-engineered ZFs are orthogonal to one another. sTF43-8 activated noncognate Promoter21-16 due to the fortuitous creation of a sequence that is significantly similar to the binding sequence of 43-8, when the downstream BamHI restriction site is considered.
interaction between ZF43.8-KRAB and 8*ZF43.8-CMV promotor
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ A synthetic biology framework for programming eukaryotic transcription functions. Khalil AS, Lu TK, Bashor CJ, ..., Joung JK, Collins JJ. Cell, 2012 Aug;150(3):647-58 PMID: 22863014; DOI: 10.1016/j.cell.2012.05.045
[edit]
Categories
Parameters
//dna
//promoter
//promoter
| None |