Coding

Part:BBa_K2762002:Design

Designed by: Ting-Shou Liu   Group: iGEM18_NCKU_Tainan   (2018-09-29)
Revision as of 13:31, 12 October 2018 by ThomasLiu (Talk | contribs) (→‎Design Notes)


Small subunit of the ribulose-bisphosphate carboxylase/oxygenase rbcS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcS sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

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References