Coding

Part:BBa_K2762001:Design

Designed by: Ting-Shou Liu   Group: iGEM18_NCKU_Tainan   (2018-09-29)
Revision as of 13:07, 12 October 2018 by ThomasLiu (Talk | contribs) (→‎Source)


Assembly chaperone of ribulose-bisphosphate carboxylase/oxygenase rbcX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first codon optimized the rbcX sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning. The sequence is then cloned into the pSB1C3 plasmid with HindIII and SpeI.


Source

Codon oprimized Synechococcus elongatus PCC7002.

References