Generator

Part:BBa_K2694000:Experience

Designed by: Jessica Larocque   Group: iGEM18_NDC-HighRiverAB   (2018-10-10)
Revision as of 23:55, 11 October 2018 by Megeorge (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2694000

User Reviews

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[http://2018.igem.org/Team:NDC-HighRiverAB NDC-HighRiverAB]

A comparison of 4-nitrophenyl palmitate and 4-nitrophenyl octanoate

Figure 1: The top row shows centrifuge tubes containing the 4-nitrohenol ester reaction mixture for the palmitate (a) and octanoate (b) esters with no cells added. The middle row shows the response for a negative control where DH5⍺ cells without any plasmid were added to reaction mixtures containing 4-nitrophenyl-plamitate (c) or 4-nitrohenyl-octanoate (d). The bottom row shows the response for the BBa_K2694000 circuit transformed into DH5⍺ cells when added to reaction mixtures containing 4-nitrohenyl-plamitate (e) or 4-nitrohenyl-octanoate (f). All reaction mixtures were 1mM of the nitrophenyl ester with 1% v/v Triton X-100 in 0.1M pH 7 phosphate buffer.

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