Help:Spring 2008 DNA distribution

Revision as of 14:21, 1 May 2008 by KCliff (Talk | contribs) (→‎Grouping of parts for QC project)

iGEM 2008 DNA Parts Kit

Background on 08 distribution


Quality Control Process

This year the Registry has undertaken extensive quality control measures in order to better ensure the accuracy of DNA parts sent to the iGEM 2008 teams. This QC process began in February and has examined each part currently in the Registry to determine if the plasmid and the part within are correct according to the documentation provided with each submission. The results for each part were then posted on the Registry's DNA repository page [ ], allowing iGEM teams to access this information before using the submissions to create their own parts.

Grouping of parts for QC project

Each part in the Registry was assigned a well in a specific 96 well staging plate according to its antibiotic resistance and its base pair length. This grouping made it easier to examine the parts against their known antibiotic resistance and to then sequence their DNA.

Streaking for Single Colonies

Instruction on usage

  • punching out spots
  • transforming
  • picking single colony
  • making glycerol
  • finding part location